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Macrophage uptake of 100nm polystyrene beadsImage by: Dr Julian Moger
CARS microscopy can be used to visualise polystyrene nanoparticles uptaken by live cells.
The figure shows 100 nm polystyrene beads in cultured macrophage cells; the vibrational signatures of the
nanoparticles and the cells can be separated by tuning (&omega p – &omegas).
The green image contrast corresponds
to the CH2 of lipids (2485 cm-1) and the red C=C vibrational stretch of polystyrene (~1600 cm-1).
This study is facilitated by the strong Raman response and high density of
CH2 groups in lipids and C=C in polystyrene, which yield strong CARS signals.