Facilities
The front-end of our system is a modified Olympus IX71 inverted microscope. Dual sources, a pico-second OPO tunable from 700 – 1400nm (Levante Emerald from APE, Berlin),
and a switchable femto- and pico-second system tunable from 700 – 1600nm (MIRA-D, and MIRA OPO from Coherent, USA)
provide the multiple contrast modalities; Multiphoton fluorescence, Second- and Third-Harmonic Generation (SHG & THG) and
Coherent Anti-stokes Raman Scattering (CARS).
| MIRA 900D
Dual femto- and pico- second operation
In femto mode provides excitation for second harmonic generation, multiphoton fluorescence, multiplex CARS and time-resolved CARS
In pico mode provides excitation for frequency modulated CARS
Output power: 1.2 W
Wavelength Range: 690 - 990 nm
Pulse width (femto mode): ~100 fs
Pulse width (pico mode): ~1 ps
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| MIRA OPO
Dual femto- and pico- second operation
In femto mode provides excitation for third harmonic generation, sum frequency generation, pump-probe microscopy
In pico mode provides excitation for frequency modulated CARS
Output power: 300 mW
Wavelength Range: 1050 - 1600 nm
Pulse width (femto mode): ~120 fs
Pulse width (pico mode): ~2 ps
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| Levante OPO
This is a picosecond OPO pumped by a frequency doubled Nd:YVO-Laser (523 nm). The signal and idler are tuneable from
690 to 990 nm and 1150 to 2300 nm respectively.
There is perfect spatial and temporal overlap of signal and idler beam, which can be outcoupled on one joint beam axis.
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| Multiphoton Imaging
A modified Olympus FV300 and IX71 allows multiphoton imaging.
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| Adjacent Cell Culture Room
Class II cell culture hood.
Incubator.
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| Adjacent wet lab facility
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