Bovine Mesenteric Lymphatics Vessel

A second harmonic generation image (left) & 2-photon autofluorescence image (right) of a bovine mesenteric collecting lymphatic vessel, stretched with an transmural pressure head. The wavelike collagen fibres can be seen to cross over one another in a 'wicker basket' type pattern. The stretched elastin fibers are clearly visible as bright straight lines, mostly in the direction of the vessel (left/right). There is a dimmer green background which is the spill over of the collagen flourescence (and can be compared directly to the SHG image).