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Left: A single input photon (blue) excites a single photon (green).
Right: Multiphoton Excitation. Two input photons (red) combine to excite a single photon (green).
As well as excitation of extraneous fluorophores, MPE can be used to excite indigenous tissue components such as NAD(P)H, flavins, and porphyrins. The figure below shows an MPE image of artery wall using elastin as the intrinsic fluorophore.
ExampleElastin in an artery wall viewed with Multiphoton Excitation